Akatirongera
Marariya ihosha yakakomba, dzimwe nguva inouraya, inotapurwa nefivha, kutonhora, uye kupererwa neropa uye inokonzerwa neutachiona hunopfuudzwa kubva kumunhu kuenda kune mumwe nokurumwa neumhutu hunonzi Anopheles.Kune marudzi mana emarariya anogona kubata vanhu: Plasmodium falciparum, P. vivax, P. ovale, uye P. malariae.Muvanhu, zvipembenene (zvinonzi sporozoites) zvinoenda kuchiropa uko zvinokura uye zvinosunungura imwe fomu, merozoites.Chirwere chacho idambudziko guru rehutano munzvimbo dzakawanda dzinopisa uye subtropics.Vanhu vanopfuura mamiriyoni mazana maviri pasi rose vane malaria.
Parizvino, marariya inoonekwa nekutsvaga utachiona mudonhwe reropa.Ropa richaiswa pachisiraidhi chemaikorosikopu uye rikasvibiswa kuitira kuti zvipembenene zvionekwe pasi pemaikorosikopu.Pazvinozvino, nyaya dzekuongororwa kwekiriniki dzine chekuita nemarariya ndiko kuwanikwa kwemasoja ekudzivirira emarariya muropa revanhu kana serum neimmunoassay.Iyo ELISA fomati uye immunochromatographic fomati (nekukurumidza) yekuona antibody yemalaria inowanikwa munguva pfupi yapfuura.
Test Principle
The Malaria Pf bvunzo ndeye immunochromatographic (nekukurumidza) bvunzo yemhando yekuona masoja emasotypes ese (IgG, IgM, IgA) zvakanangana nePlasmodium falciparum nePlasmodium vivax panguva imwe chete muserum yemunhu, plasma kana ropa rose.
Main kuumbwa
1. Test card 2. Dombo donje pad rinoraswa 3. Tsono yekuunganidza ropa inoraswa 4. Diluent
Mamiriro ekuchengetedza uye kushanda
1.Chengetedza pa4℃～40℃,nguva yechokwadi inomisikidzwa nemwedzi makumi maviri nemana.
2.After kuvhura aluminium foil bag, kadhi rekuedza rinofanira kushandiswa nokukurumidza sezvinobvira mukati memaminitsi makumi matatu.Sample diluent inofanira kuvharwa pakarepo mushure mekuvhurwa uye kuiswa munzvimbo inotonhorera.Ndokumbira uishandise mukati menguva yechokwadi.
Muenzaniso Chikumbiro
1. ropa rose : Unganidza ropa rose uchishandisa anti-coagulant yakakodzera.
2. serum kana plasma: Centrifuge ropa rose kuti uwane plasma kana serum specimen.
3. Kana zvienzaniso zvikasakurumidza kuongororwa zvinofanirwa kuiswa mufiriji pa2 ~ 8°C.Kwenguva dzekuchengetedza dzinopfuura mazuva matatu, kutonhora kunokurudzirwa.Dzinofanirwa kuunzwa kune tembiricha yekamuri isati yashandiswa.
4. Mienzaniso ine precipitate inogona kupa mhedzisiro yebvunzo isingaenderane.Mienzaniso yakadaro inofanira kujekeswa isati yaongororwa.
5. Ropa rose rinogona kushandiswa kuongororwa nekukurumidza kana kuchengetwa pa2 ~ 8°C kusvika kumazuva matatu.
Testing method
Ndokumbira uverenge murayiridzo Wekushandisa zvakanyatsonaka usati waedza.Samples dzinofanirwa kuongororwa, mareagents ekuona uye zvimwe zvinhu zvinoshandiswa pakuyedza zvinofanirwa kuenzana nekamuri tembiricha.Muedzo unofanira kuitwa pakamuri tembiricha.
1.Bvisa kadhi rekuongorora bepa nekubvarura bhegi realuminium foil, uye riise pasi panzvimbo yekushanda.
2.Kutanga shandisa pipette yepurasitiki kuti ushuvire 1 donho reropa rose, serum kana plasma sampuli (inenge 10μ1) mumuenzaniso wetsime (S) yekadhi rekuedza.Wobva wawedzera madonhwe maviri kusvika matatu (angangoita 50 kusvika ku100 μl) yemuenzaniso dilution.
3.Observe the experimental results within 5-30 maminitsi (zvigumisiro hazvina maturo mushure memaminitsi makumi matatu).
Chenjero: Nguva yekududzira iri pamusoro yakavakirwa pakuverenga mhinduro dzebvunzo pakamuri tembiricha ye15 ~ 30°C.Kana tembiricha yekamuri yako yakadzika zvakanyanya kupfuura 15 ° C, saka nguva yekududzira inofanira kuwedzerwa zvakakodzera.
Kuturikira mhinduro dzebvunzo
Zvakanaka: Mutsara weruvara munharaunda yekutonga (C) inooneka uye mutsara weruvara unoonekwa munharaunda yebvunzo (T).Mhedzisiro yacho yakanaka.
Negative: Mutsetse weruvara mudunhu rekutonga (C) unoonekwa uye hapana mutsara weruvara unoonekwa munharaunda yebvunzo (T) Mhedzisiro yacho haina kunaka.
Haina basa: Hapana mutsara wakaonekwa munharaunda yeC.
Haina basa: Hapana mutsara wakaonekwa munharaunda yeC.
Kuganhurirwa kwenzira dzekuongorora
1. Kuongororwa kunogumira pakuonekwa kwemasoja ekudzivirira chirwere cheMalaria zvese Plasmodium falciparum nePlasmodium vivax panguva imwe chete.Kunyangwe bvunzo iri chaiyo mukuona masoja ekudzivirira chirwere kuMalaria Pf, chiitiko chakaderera chemhedzisiro yenhema chinogona kuitika.Mimwe miedzo yekiriniki inowanikwa inodiwa kana mhinduro dzisina chokwadi dzawanikwa.Sezvinei zvese zvekuongororwa kwekuongorora, mhedzisiro yekiriniki yekuongororwa haifanirwe kubva pamhedzisiro yebvunzo imwe chete, asi inofanira kungoitwa nachiremba mushure mekunge zvese zvekiriniki nerabhoritari zvakaongororwa.
2. Mhedzisiro yemhedzisiro yechigadzirwa ichi inodudzirwa nemeso emunhu, uye inotapukirwa nezvinhu zvakaita semhosho yekuongorora yekuona kana mitongo yekutonga.Nokudaro, zvinokurudzirwa kudzokorora kuedza apo ruvara rwebhandi harisi nyore kuziva.
3. Iyi reagent ndeye qualitative discovery reagent.
4.Iyi reagent inoshandiswa pakuona serum yega, plasma kana sampuli dzeropa rose.Usashandise pakuona mate, weti kana mamwe mvura emuviri
PERFORMANCE CHARACTERISTICS
1. Sensitivity uye Zvakananga:Muedzo weMalaria Pf wakaedza nemasampuli ekiriniki akanaka uye asina kunaka akaedzwa nekuongorora diki kweropa rose.
Malaria Pf evaluation results
| Reference | Malaria Pf | Total Results | ||
| Nzira | Mhedzisiro | Zvakanaka (T) | Negative | |
| microscopic kuongorora | Pf Positive | 150 | 20 | 170 |
| Pf Negative | 3 | 197 | 200 | |
| Total Results | 153 | 217 | 370 | |
Mukuenzanisa bvunzo yeMalaria Pf zvichienderana nekuongorora diki kweropa rose, mhedzisiro yakapa kunzwisisika kwe88.2% (150/170), chaiyo ye98.5% (197/200), uye chibvumirano chese che93.8% (347/370) .
2. Kururama
Mukati mekumhanya chaiko kwakatemwa nekushandisa gumi replicates ezvienzaniso zvina zvakasiyana zvine maratidziro akasiyana emasoja ekudzivirira chirwere.Izvo zvisina kunaka uye zvakanaka zvakakosha zvakanyatsozivikanwa 100% yenguva.
Pakati pekuita nemazvo kwakatemwa nekushandisa zvienzaniso zvina zvakasiyana zvine maantibody akasiyana muzvikamu zvitatu zvakasiyana zvine matatu akasiyana emidziyo yekuyedza.Zvakare mhedzisiro yakaipa uye yakanaka yakaonekwa 100% yenguva.
PROCAUTION
1. Yekushandisa in vitro diagnostic chete.
2. Usadya kana kuputa paunenge uchibata sampuli.
3. Pfeka magirovhosi ekudzivirira paunenge uchibata mienzaniso.Geza maoko zvakakwana pashure.
4. Dzivisa kupuruzira kana kuumbwa kweaerosol.
5. Chenesa zvinorasika zvakanaka uchishandisa mushonga unouraya utachiona wakakodzera.
6. Bvisa uye bvisa zvienzaniso zvose, maitirwo ekugadzirisa uye zvinhu zvinogona kusvibiswa, sekunge imarara ane utachiona, mumudziyo webiohazard.
7. Usashandise test kit kana homwe yakuvadzwa kana chisimbiso chatyoka.
【Indekisi yeEC Symbols】
